【摘要】 目的探讨骨质疏松SD大鼠颅骨中IL-10的表达与成骨细胞内雌激素受体表达的相关性。方法 40只SD雌性大鼠用随机数字表法随机分为对照组20只和去势(去卵巢)组20只(去势第5周组、第7周组各10只),用双能X线吸收法测定各组骨密度,用免疫组织化学ABC法及图像分析技术观察各组颅骨组织中IL-10表达;用二次酶消化、反复贴壁法分离纯化培养大鼠颅骨成骨细胞,并用钙-钴法碱性磷酸酶(ALP)染色法及Ⅰ型胶原免疫组化染色法观察、鉴定大鼠成骨细胞。应用半定量Western blot法检测各组SD大鼠成骨细胞内雌激素受体。结果去势组骨密度明显低于对照组(P<0.01);去势组骨小梁周边IL-10阳性成骨细胞数明显少于对照组(P<0.01),并且染色较对照组浅。ALP染色、Ⅰ型胶原免疫组化及形态学观察符合成骨细胞特征。去势组雌激素受体表达明显低于对照组,去势第7周低于去势第5周(P<0.01)。结论雌激素受体的表达与成骨细胞IL-10表达水平成正相关。更多还原

【Abstract】 Objective To observe the correlation of estrogen receptor in osteoblasts and IL-10 expression of skull in osteoporosis rats.Methods 40 SD rats were randomly divided into control group(n=20)and ovariectomized group(n=20,5 weeks 10 rats,7 weeks 10 rats).The bone mineral density was measured by ray absorptiometry,IL-10 expression in the skull were detected by immunohistochemical staining of SABC method and the image analysis techniques;The rat osteoblasts were purified by two-step enzymatic digestion method and successive adherence method.The osteoblasts were observed by calcium alkaline phosphatase cobalt method(ALP)and collagen type Ⅰ immunohistochemical staining method.The estrogen receptors of osteoplasts in SD rats were identified by semi-quantitative western blot.Results The ovariectomized group showed less bone mineral density(P<0.01);The IL-10 positive osteoblasts in the trabecula of ovariectomized group was less than that of control group(P<0.01),and their staining was lighter than control group;the observable results by ALP and collagen type Ⅰ immunohistochemical method corresponded to the morphology of osteoblast features.The estrogen receptor expression of ovariectomized group was significantly lower than that of control group,that of the 7th week was significantly lower than the 5th week in the ovariectomized group(P<0.01).Conclusion Estrogen receptor expression is positively correlated with the expression level of IL-10 in the osteoblasts.更多还原