【摘要】 目的探讨多环芳烃(Polycy clic aromatic hydrocarbons,PAHs)相关肺癌患者血浆谷光甘肽(GSH-Px)、丙二醛(MDA)、P21蛋白水平与氧化应激的关系,为PAHs相关肺癌发病机制及诊断指标的研究提供参考依据。方法实验对象分为PAHs相关肺癌者(肺癌组31人)、非肿瘤性肺病者(配比组34人)和健康者(健康组33人),采用二巯代硝基苯甲酸比色法(DTNB法)、硫代巴比妥法(TAB法)、蛋白质印迹法(Western Blot)分别检测受试者血浆中GSH-Px活力、MDA含量及P21蛋白水平。结果血浆中GSH-Px活力呈现肺癌组<配比组<健康组的梯度(P<0.05);与配比组和健康组比较,肺癌组MDA含量及P21蛋白水平明显增高(P<0.05)。在肺癌组中,MDA含量及P21蛋白水平与GSH-Px呈负相关(分别为r=-0.2269,P<0.01和r=-0.1517,P<0.05),P21与MDA呈正相关(r=0.2539,P<0.01)。结论 PAHs相关肺癌可能与氧化应激密切相关;联合检测GSH-Px、MDA、P21蛋白水平可能有助于PAHs相关肺癌的早期诊断。更多还原

【Abstract】 Objective To explore the relationship between serum levels of glutathione peroxidase(GSH-Px),malonydialdehyde(MDA)and P21in PAHs induced lung cancer and its possible incidence mechanism and diagnostic criteria.Methods DTNB,TAB and Western blot were used respectively to detect the serum level of GSH-Px,MDA and P21 protein in patients of lung cancer group(31 people),matched group(34 people)and healthy group(33 people).Results Results of GSH-Px activity showed in the order of lung cancer group<matched group<healthy group,the differences were Significant(P<0.05).The levels of MDA and P21 protein were significantly higher in cancer group than that in matched and healthy group(P<0.05).In the cancer group,the level of GSH-Px was negatively correlated with MDA and P21(r=-0.1520,P<0.05 and r=-0.1517,P<0.05)and positively correlated with serum levels of MDA and P21(r=0.2539,P<0.01).Conclusions Oxidative stress might be closely associated with PAHs induced lung cancer.Combined detection of GSH-Px,MDA and P21 might be helpful for the early diagnosis of PAHs induced lung cancer.更多还原