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适冷溶菌酶产生茵的分子鉴定及其酶学性质的研究

Molecular identification of cold-active lysozyme-producing strain and enzyme characteristics

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【Author】 WANG Quan-fu~(1,2),HOU Yan-hua~2,SHEN Ji-hong~3,LIN Xue-zheng~3,YAN Pei-sheng~2,LI Ning~1(1.Department of Applied Chemistry, Harbin Institute of Technology,Heilongjiang Harbin,150001;2.School of Ocean,Harbin Institute of Technology at Weihai,Shandong Weihai,264209 3.Key Laboratory of Marine Bio-active Substances,First Institute of Oceanorgarphy,State Oceanic Administration,Shandong Qingdao 266061)

【机构】哈尔滨工业大学化工学院；哈尔滨工业大学威海校区海洋学院；国家海洋局第一研究所海洋生物活性物质重点实验室；

【摘要】采用比浊法对本试验室保存的126株南极微生物产适冷溶菌酶进行测定,发现7株菌产溶菌酶。并对其中的菌株NJ147进行ITS-5.8 S rDNA基因序列的同源性和系统发育分析,结果表明:菌株NJ147属于Debaryomyces hansenii属。该菌株所分泌溶菌酶的最适作用温度为30℃,在0℃时酶活性是最高活性的32.6%;对热敏感,其半衰期为40℃保温30 min;最适pH为6.0,是一种典型的适冷酶。该酶对6种受试菌株均有不同程度的抑制,具有广谱杀菌谱,具有潜在的工业应用价值。更多还原

【Abstract】 Seven stains producing cold-active lysozyme were serened from 126 strains of antarctic microorganisms using nephelometry assay.The ITS-5.8S rDNA gene sequences homology and phylogenetic analysis showed that the strain NJ147 belonged to the genus Debaryomyces hanseniis.The lysozyme characteristics were studied.The optimum lysozyme activity was observed at 30 ℃ and about 32.6 % of the optimum activity was still detectable at 0 ℃.It was thermal lability,the enzyme t_i was at 40 ℃ for 30 min.The optimum enzyme activity was observed at pH 6.This lysozyme showed typical features of a cold-adapted enzyme.It had broad-spectrum against 6 kinds of strains and had potential in industry.更多还原

【关键词】适冷溶菌酶；南极微生物； ITS-5.8 S rDNA； Debaryomyces hanseni；
【Key words】 cold-active lysozyme； antarctic microorganisms； ITS-5.8S rDNA； Debaryomyces hanseni；

【基金】国家863计划资助项目(2007AA0905);中国博士后科学基金(20090451004);黑龙江省博士基金;山东省自然科学基金(Y2007D58,ZR2009DQ023);威海市科技攻关项目(2008087);哈尔滨工业大学优秀创新团队支持计划和科研创新基金(HIT-NSRIF201011)资助

【文献出处】工业微生物 ,Industrial Microbiology , 编辑部邮箱 ,2011年03期

【分类号】Q93;TQ920.1

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