【摘要】 利用pPICZαA作为新的表达载体和表达宿主酵母Gs115,成功地构建了新的人源过氧化氢酶表达工程菌G13,并考察了新的工程菌G13的遗传稳定性。通过对新的重组菌整合质粒的酶切,PCR鉴定,及SDS-PAGE电泳、单抗dot-blot证实了该重组菌质粒构建正确,可以有效表达重组的人源过氧化氢酶。新的重组酵母菌G13在连续传代24次后,没有发生质粒的丢失现象,表达的过氧化氢酶活力稳定。更多还原

【Abstract】 Using pPICZαA as the new yeast expressing vector,the genetic engineering strain GS115-pPICZαA CAT producing human catalase was successfully constructed,and the genetic stability of the strain G13 was studied.Restriction endonuclease digestion and PCR analysis showed that the recombinant plasmid was properly constructed,and the SDSPAGE convinced that the strain could express the target protein.The recombinant strain G13 was continuously incubated for 24 generations.The results showed the plasmid had structural stability and the recombinant strain could maintain human catalase activity.更多还原