【摘要】 目的研究寡核苷酸适配子对动脉粥样硬化病变组织结合的特异性。方法将前期研究获得的特异性适配子命名为PM2,FITC标记后,荧光显微镜分别观察适配子与血管平滑肌细胞、巨噬细胞、内皮细胞、巨噬细胞源性泡沫细胞结合情况。采用高脂方法建立兔的动脉粥样硬化模型。利用PCR方法和荧光显微镜观察适配子与动脉粥样硬化病变结合情况。结果 PM2适配子与巨噬细胞源性泡沫细胞高度特异性结合,但不与血管平滑肌细胞、巨噬细胞、内皮细胞结合;PM2适配子能特异性结合动脉粥样硬化斑块组织,而不结合正常动脉组织结合。结论筛选出的PM2适配子特异性结合巨噬细胞源性泡沫细胞和动脉粥样硬化病变。更多还原

【Abstract】 Objective To observe the binding specificity of aptamers and THP-1 macrophage derived foam cells and atherosclerotic lesions.Methods The FITC-labeled No 40 aptamers(PM2),binding to THP-1 macrophages,vascular smooth cells,endothelial cells and THP-1 macrophage derived foam cells were observed by fluorescence microscopy respectivly.Model of atherosclerosis was formed by high-fat feeding rabbit methods.The binding of aptamers and atherosclerotic lesions were detected by fluorescence microscopy or PCR.Results The PM2 aptamers didn’t bind to THP-1 macrophages,vascular smooth cells and endothelial cells but bind to THP-1 macrophage derived foam cells and atherosclerotic lesions.Conclusion We have obtained PM2 aptamers specifically binding to THP-1 macrophage derived foam cells and atherosclerotic lesions.更多还原