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罗汉果转抗病基因NPR1的研究

Transferring disease resistance gene NPR1 into Siraitia grosvenorii

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【作者】 杨华李惠敏覃屏生高成伟秦新民

【Author】 YANG Hua,LI Hui-Min,QIN Ping-Sheng,GAO Cheng-Wei,QIN Xin-Min(College of Life Science,Guangxi Normal University,Guilin 541004,China)

【机构】 广西师范大学生命科学学院

【摘要】 以罗汉果子叶为外植体,研究了影响根癌农杆菌介导的罗汉果遗传转化的若干因素,建立了罗汉果遗传转化体系。结果表明,5 d苗龄的子叶、预培养1 d、侵染20 min、共培养4 d、共培养温度22℃、AS100μmol/L、Hy浓度不定芽筛选为10 mg/L,生根筛选为20 mg/L转化率最高。抗性苗经PCR和Southern印迹分析,初步证实NPR1基因已整合至罗汉果基因组中,转化率为1.5%。

【Abstract】 Using cotyledon of Siraitia grosvenorii as explants,an efficient and reliable transformation system was established.Several factors affecting genetic transformation of Agrobacterium-mediated S.grosvenorii were studied.The results showed that 5 days aged cotyledon using for infection explants,pre-culture 1 d,bacterial infection time 20 min,co-culture 4 d with 22 ℃,AS concentrate 100 μmol/L,10 mg/L of hygromycin was suitable screening concentration for the differentiation of adventitious bud and root could get the better transformation efficiency under 20 mg/L of hygromycin.The transformants were confirmed by PCR and Southern blotting analysis with the transformation rate 1.5%.

【基金】 广西自然科学基金(桂科自0728095)~~
  • 【分类号】Q943.2
  • 【被引频次】13
  • 【下载频次】237
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