【摘要】 目的:通过比较广西北回归线两侧的南宁地区和桂林地区人群肝组织黄曲霉毒素B1(Aflatoxin B1,AFB1)的暴露水平,探讨AFB1暴露与原发性肝细胞癌(Hepatocellular carcinoma,HCC)发生的关系。方法:应用免疫组化法检测南宁和桂林地区共165例HCC患者和95例正常肝组织中AFB1-DNA加合物的表达水平。结果:AFB1-DNA加合物阳性率分别为:南宁和桂林地区HCC组分别为59.5%(47/79)与17.4%(15/86)(χ2=31.042,P<0.001);南宁与桂林地区对照组分别为15.5%(9/58)和10.8%(4/37)(χ2=1.632,P=0.121);南宁地区HCC组59.5%(47/79)与对照组15.5%(9/58)间阳性率差异有统计学意义(χ2=21.484,P<0.001),桂林地区HCC组17.4%(15/86)与对照组10.8%(4/37)间阳性率无统计学差异(χ2=0.318,P=0.573)。结论:广西AFB1暴露水平存在区域性差异,南宁地区AFB1暴露水平要远高于桂林地区。AFB1暴露是南宁地区HCC高发的危险因素。更多还原

【Abstract】 Objective:To explore the association between aflatoxin B1(AFB1) exposure and the development of hepatocelluar carcinoma(HCC).Methods:Immunohistochemistry method was used to detect the levels of AFB1-DNA adducts.A total of 260 cases of HCC and non-HCC liver tissues were categorized into 4 groups according to their residency,HCC group(n=79) and controls(n=58) from Nanning area,HCC group(n=86) and controls(n=37) from Guilin area.Results:The AFB1-DNA adduct levels from Nanning area was significantly higher than those from Guilin area(χ2=31.042,P<0.001).The AFB1-DNA adduct levels of HCC and control groups from Nanning area were different(χ2=21.484,P<0.001),but there was no difference in AFB1-DNA adduct levels between HCC and control groups from Guilin area(χ2=0.318,P=0.573).Conclusion:There is a regional difference in AFB1 exposure in Guangxi,the AFB1-DNA adduct levels from Nanning area are much higher than those from Guangxi area.The AFB1 exposure is a risk factor for the hepatocarcinigenesis of HCC in Nanning area.更多还原