【摘要】 目的:观察甘露醇预处理对骨髓间充质干细胞(BMSCs)静脉移植治疗血管性痴呆模型大鼠行为学的影响。方法:以双侧颈总动脉永久性结扎法(2-VO)制作大鼠血管性痴呆模型。造模成功后,随机分成培养基组8只(注射1 mL无血清培养基)、BMSCs移植治疗组11只(注射1×106个BMSCs 1 mL)及甘露醇预处理BMSCs移植治疗组9只(注射1.5 g/kg甘露醇后,在10~30 min之内注射1×106个BMSCs 1 mL)。通过Morris水迷宫实验检测血管性痴呆大鼠模型移植治疗前与移植4周后空间学习记忆能力,比较各组之间差异。结果:与培养基组比较,BMSCs移植治疗组和甘露醇预处理BMSCs移植治疗组大鼠空间学习记忆能力都有改善,模型大鼠逃避潜伏期缩短(P<0.05),平台象限区滞留时间延长(P<0.05);与BMSCs移植治疗组比较,甘露醇预处理BMSCs移植治疗组大鼠空间学习记忆能力改善更加明显,逃避潜伏期缩短(P<0.05),平台象限区滞留时间延长(P<0.05)。结论:甘露醇预处理后BMSCs静脉移植组治疗血管性痴呆与单独BMSCs静脉移植组大鼠比较,学习记忆能力显著改善。其机制可能与甘露醇能开放血脑屏障(BBB),增加进入脑内的BMSCs数量相关。更多还原

【Abstract】 Objective:To investigate the use of mannitol intravenous injection for bone marrow mesenchymal stem cells(BMSCs) transplantation and its effect on changes of behavior in vascular dementia rat model.Methods:Rats of experimental group were treated with a permanent bilateral common carotid arteries(CCA) occlusion(2-VO) to establish vascular dementia model.After the modeling,experimental animals were divided into culture media group(intravenously injecting serum-free growth medium 1 mL),BMSCs transfer group(intravenously injecting 1 million BMSCs in 1 mL growth medium) and mannitol+BMSCs transfer group(intravenous injection of mannitol 1.5 g/kg in PBS followed by intra-arterial injection of 1 million BMSCs in 1 mL growth medium).The changes of rats’ behaviors were detected by Morris water maze in four groups 4 weeks after transplantation.Results:Comparing to culture media group,the escape latency decreased significantly(P<0.05) and platform quadrant residence time increased significantly(P<0.05) in BMSCs transfer group and mannitol + BMSCs transfer group.Comparing to BMSCs transfer group,the escape latency decreased significantly(P<0.05) and platform quadrant residence time increased significantly(P<0.05) in mannitol+BMSCs transfer group.Conclusion:The combination of mannitol and BMSCs transplantation significantly increased learning and memory function in vascular dementia in rats,and the mechanism associated with mannital’s liberalizing BBB and facilitating the migration of BMSCs into ischemic brain tissue and its survival.更多还原