【摘要】 在超声波辅助下,以质量分数为80%的乙醇水溶液对十大功劳果实进行提取,浓缩后得到提取物。在对提取物的DPPH.、ABTS+.清除能力进行研究的基础上,探讨溶剂效应对提取物DPPH.清除活性的影响,并利用荧光猝灭法研究提取物与Cu2+、Fe2+的螯合作用。结果表明,十大功劳果实提取物清除ABTS+.和DPPH.的IC50值均远远小于10 g/L,说明提取物具有良好的自由基清除活性;溶剂的极性对提取物DPPH.清除活性具有重要影响;提取物与Cu2+、Fe2+具有较好的螯合作用,猝灭常数Ksv分别为7.57×102和3.09×103L.mol-1。更多还原

【Abstract】 Mahonia bealei fruit was extracted using ultrasonic wave to get extract.Based on the study of scavenging activities of extract against DPPH· and ABTS+·,the influence of solvent effect on scavenging activities was studied and the fluorescence-quenching spectroscopy was employed to study their chelation abilities with copper(Ⅱ) and ferrous ion.The results showed that IC50 of the extract of Mahonia bealei fruit scavenging against DPPH· and ABTS+· were much lower than 10 g/L,implying that the extract had good scavenging activities,while the solvent effects had important influence on scavenging activities.In addition,the extract of Mahonia bealei fruit showed good chelation abilities with copper(Ⅱ) and ferrous ion,with the quenching constants Ksv 7.57×102 and 3.09×103 L·mol-1,respectively.更多还原