【摘要】 以南极假丝酵母脂肪酶(Candida antarctica lipase B)为催化剂、葡萄糖和棕榈酸乙烯酯为原料,在离子液体中合成葡萄糖棕榈酸酯。并研究了最佳反应条件,结果表明,以离子液体1-丁基-3-甲基咪唑三氟甲磺酸盐([Bmim][TfO])为介质的最佳条件为:温度40℃、酶浓度为50mg/mL、糖酯摩尔比1∶3。产物用傅里叶变换红外光谱(FT-IR)、高效液相色谱(HPLC)、质谱(MS)和核磁共振(NMR)进行了表征,结果表明是6-O-葡萄糖棕榈酸酯。更多还原

【Abstract】 In the synthesis of palmitolyglucose ester,Candida antarctica lipase B was used as catalyst for the synthesis of palmitolyglucose ester by glucose and palmitic acid vinyl ester in ionic liquids.The effects of substrate ratio,lipase content and temperature were studied.The highest yield of sugar ester was obtained in under such conditions as the reaction temperature of 40 ℃,the enzyme concentration of 50 mg/mL,the mole ratio of glucose /vinyl palmitate of 1∶ 3.The structure of products was characterized by FT-IR,HPLC,MS and NMR.The results showed that the product is 6-O-palmitolyglucose ester.更多还原