【摘要】 从猪粪便中分离得到一株新饲用乳杆菌,对其16S rDNA基因进行了测序,该段基因序列已提交GenBank,登录号为HQ641209。将该菌株16SrDNA基因序列进行在线BLAST比对并与其他乳杆菌属菌株的16S rDNA序列建立系统发育树,结果显示该菌16SrDNA序列与NCBI公布的约氏乳杆菌Lactobacillus johnsonii(AB295648)的同源性为99.7%,因此该菌鉴定为约氏乳杆菌(Lactobacillus johnsonii),将其命名为"Lactobacillus johnsonii Sun001"。经测试,该菌株对E.coli K88、K99菌株的生长有抑制作用,在pH为2.5的模拟胃液中处理3h存活率为68.8%,在0.3%胆盐浓度的模拟肠液中处理3h存活率为21.4%。更多还原

【Abstract】 A new Lactobacillus was isolated from a faecal sample of pig.The 16S rDNA gene was sequenced,and it was submitted to GenBank,the accession number was HQ641209.Online BLAST with other Lactobacillus and phylogenetic analysis based on the fragment of 16S rDNA reveal that its sequence homology was 97.9% comparing with Lactobacillus johnsonii strain(AB295648).The isolated strain was identified as Lactobacillus johnsonii and named Lactobacillus johnsonii Sun001.L.johnsonii had high level tolerance to the simulated gastric juice(pH 2.5,3 h of incubation,survival rate was 68.8%) and simulated gastric juice(bile salt concentration 3%,3 h of incubation,survival rate was 21.4%) and high antibacterial activity to E.coli K88,K99.更多还原