【摘要】 本试验旨在检查中药颗粒剂对传染性法氏囊病(IBD)鸡免疫器官损伤的修复作用。采用病理组织学方法检查传染性法氏囊病自然耐过鸡和用中药颗粒剂治疗而康复鸡的免疫器官,采用光学显微镜对其主要免疫器官进行病理组织学观察。即随机抽取中药颗粒剂治疗组和自然耐过对照组25日龄鸡各10只,其中中药治疗康复后35日龄鸡10只,自然耐过35日龄鸡7只,死亡3只。扑杀被检鸡,采集各鸡法氏囊、脾脏和肝脏病料,眼观病变以后,用10%中性福尔马林固定,石蜡包埋,切片,苏木素-伊红染色,组织切片,显微镜检查病理组织学变化,并进行显微组织拍照直观比较自然耐过鸡和自制中药颗粒治疗康复鸡的免疫器官病理组织学变化。被检的IBD自然耐过鸡的免疫器官仍呈轻度病理性变化,而采用自制中药颗粒治疗痊愈鸡的病理变化基本一致,表现为免疫增强。中药颗粒剂能够显著修复IBD鸡免疫器官的损伤。更多还原

【Abstract】 This experiment was conducted to assess the rehabilitation of immune organ of affected chicken for infectious bursal disease(IBD) was repaired by herbal-medicine pellets.The recovered chickens infected by IBD of control group not using drug and treatment group using the granular formulation of herbal-medicine pellets were killed,and their immune organs were performed to evaluate the histopathology change under the microscope.Ten twenty-five-day chickens were selected at random from control group not using drug and treatment group using the granular formulation of herbal-medicine pellets respectively.After ten days,all selected chickens from treatment group were recovered,whereas three of ten chickens from treatment group were died.Bursa of Fabricius,spleen and liver of each chicken were collected,observed.The tissues of these organs were fixed using 10% Formalin solution,embedded by paraffin.Embedded tissues were sliced up,stained and then observed.The histopathology photographs were conducted to assess the pathology change of immune organ from recovered chickens infected by IBD of control group not using drug and treatment group using the granular formulation of herbal-medicine pellets.Histopathology change was low grade in control group,where as no histopathology change was observed in treatment group.Immunoenhancement was showed in treatment group.Impairment of immune organ of affected chicken for IBD can be repaired markedly using the granular formulation of herbal-medicine pellets.更多还原