【摘要】 目的探讨肝抗原自身抗体检测对自身免疫性肝病(ALD)的诊断价值。方法分别采用间接免疫荧光法和免疫印迹法检测51例ALD患者、85例各类病毒性肝炎和其他肝病患者血清中的抗核抗体(ANA)和肝抗原自身抗体。结果 51例ALD患者中,自身免疫性肝炎(AIH)组患者血清中检出的ANA、抗LKM-1抗体、抗SLA抗体阳性率分别为87.4%、27.8%和29.4%;原发性胆汁性肝硬化(PBC)组中ANA、抗线粒体抗体(AMA-M2)、抗Sp100抗体和抗gp210抗体阳性率分别为85.2%、92.6%、33.3%和25.9%;原发性硬化性胆管炎(PSC)组中ANA阳性率为18.6%,其他各项检测指标均为阴性;对照组ANA阳性率为11.6%,其他检测指标均为阴性。结论肝抗原自身抗体检测对ALD的明确诊断和分类有重要的临床意义。更多还原

【Abstract】 Objective To evaluate the diagnostic value of antoantibody against liver antigens in autoimmune liver disease.Methods Serum samples were tested for ANA by indirect immunofluo-rescence assay(IIF)and auto-antibody of liver antigens by Western-blot(WB)in 51 cases of patients with autoimmune hepatic diseases and 85 cases with various virus hepatitis and other liver diseases.Results The positive rates of ANA,anti-LKM-1,anti-SLA were 87.4%,27.8% and 29.4% in autoimmune hepatitis(AIH).The positive rates of ANA,anti-AMA-M2,anti-Sp100,anti-gp210 were 85.2%,92.6%,33.3% and 25.9% in primary biliary cirrhosis(PBC).The positive rate of ANA was 18.6% and others were negative in primary sclerosing cholangitis(PSC).The positive rate of ANA was 12.9% and others were negative in control group.Conclusion negative detection of autoantibody against liver antcgens is useful clinically for diagnosing and classifying liver diseases.更多还原