【摘要】 目的:观察神经病理性疼痛大鼠脊髓背角Ⅱ层突触形态结构的可塑性,探讨神经病理性疼痛的发病机制。方法:SD大鼠15只,随机分为正常组、假手术组和模型组各5只,采用坐骨神经慢性压迫性损伤(CCI)建立大鼠神经病理性疼痛模型。应用透射电镜观察和体视学定量分析脊髓Ⅱ层突触形态结构的参数变化。结果:与正常组及假手术组比较,模型组大鼠脊髓背角Ⅱ层的突触后致密物质增厚(P<0.05),突触间隙增宽及突触界面曲率明显增大(P<0.05),凹型棘突触的数密度显著增高(P<0.01),总突触、棘突触和穿孔性突触的数密度增高明显(P<0.05)。结论:脊髓Ⅱ层突触形态结构可塑性变化可能参与神经病理性疼痛的发生。更多还原

【Abstract】 Objective:To explore the mechanisms of neuropathic pain by observing the ultrastructure of synapses in lamine Ⅱ of spinal dorsal horn of rats with neuropathic pain.Methods: Fifteen SD rats were randomly divided into groups normal,sham-operation and model(n=5).In the model group,chronic constriction injury of the sciatic nerve was used to establish neuropathic pain model.The changes of synaptic structural parameters in the lamine Ⅱ of the spinal dorsal horn were studied under transmission electron microscopy and morph metric analysis.Results: When compared with those of rats in sham-operation and control groups,the synaptic parameters in the lamineⅡ of the spinal dorsal horn of rats in the model group including the thickness of the postsynaptic density(PSD),the width of the synaptic cleft and the curvature of the synaptic interface were notably increased(P<0.05).Similarly,the numerical density of perforated synapses,negative synapses,spine synapses and all synapses in the model group were significantly increased relative to those in the controls(P<0.05).Conclusion: Variable configuration of synapses in lamine Ⅱ of the spinal dorsal horn may contribute to the development of neuropathic pain.更多还原