【摘要】 目的:研究胰岛素样生长因子Ⅱ(IGF-Ⅱ)、表皮生长因子(EGF)在含葡萄糖、胰岛素的CZB培养液中对ICR小鼠早期胚胎体外发育的影响。方法:①在含有葡萄糖、胰岛素的CZB培养液中分别添加0,0.1,1,10,100μg/L的IGF-Ⅱ,体外培养观察其对ICR小鼠1-细胞胚胎的作用,并设单独CZB培养液对照组。②在含葡萄糖、胰岛素和0.1μg/L IGF-Ⅱ的CZB培养液中分别添加0,0.1,1,10,100μg/LEGF,体外培养观察其对ICR小鼠1-细胞胚胎的作用,并设单独CZB培养液对照组。比较各组囊胚率、孵化率和胚胎细胞数的变化。结果:①0μg/LIGF-Ⅱ组囊胚率、孵化率及囊胚细胞数高于对照组(P<0.05)。100μg/L IGF-Ⅱ组囊胚率、孵化率及囊胚细胞数低于0,0.1,1,10μg/L IGF-Ⅱ各组(P<0.05);0.1μg/LIGF-Ⅱ组囊胚细胞数最高(P<0.05)。②0μg/L EGF组囊胚率、孵化率及囊胚细胞数高于对照组(P<0.05)。0.1,1,10μg/L EGF各组囊胚率、孵化率高于0,100μg/L EGF组(P<0.01);0.1μg/L EGF组孵化率最高(P<0.05),囊胚细胞数高于0μg/LEGF组(P<0.01);100μg/LEGF组囊胚细胞数最低(P<0.05)。结论:①1,10μg/LIGF-Ⅱ和葡萄糖及胰岛素相互作用对胚胎细胞无明显抑制或促进作用。②1,10μg/LEGF参与葡萄糖、胰岛素、IGF-Ⅱ对胚胎细胞分化的调节,却无增殖作用。③0.1μg/L IGF-Ⅱ和EGF作为胚胎培养浓度较佳。④100μg/LIGF-Ⅱ和EGF对胚胎有抑制作用。更多还原

【Abstract】 Objective: To investigate the effection of IGF-Ⅱ and EGF on the development of ICR mouse preimplantation embryos in the CZB culture medium with glucose and insulin in vitro.Methods: ①Experiment1: One-cell ICR mouse embryos were cultured in the CZB medium with glucose and insulin supplemented with IGF-Ⅱ(0,0.1,1,10 and 100 μg/L),and a separate control group of CZB medium was established.②Experiment 2: One-cell ICR mouse embryos were cultured in the CZB medium with glucose,insulin and IGF-Ⅱ(0.1 μg/L) supplemented with EGF(0,0.1,1,10 and 100 μg/L),and a separate control group of CZB medium was established.Blastocyst rates,hatched blastocyst rates and the cell numbers of blastocysts were counted.Results: ①Experiment1: IGF-Ⅱ(0 μg/L) increased the rate of blastocyst,hatched blastocyst and the total cell numbers of blastocyst compared with control group(P<0.05).IGF-Ⅱat a dose of 100 μg/L decreased the rate of blastocyst,hatched blastocyst and the total cell numbers of blastocyst compared with the other groups(P<0.05).IGF-Ⅱ at a dose of 0.1 μg/L greatly increased the total cell numbers of blastocyst(P<0.05).②Experiment 2: EGF(0 μg/L) increased the rate of blastocyst,hatched blastocyst and the total cell numbers of blastocyst compared with control group(P<0.05).EGF at doses of 0.1,1 and 10 μg/L increased the rate of blastocyst and hatched blastocyst compared with EGF at doses of 0 and 100 μg/L(P<0.05),and EGF at a dose of 0.1 μg/L had the highest rate of hatched blastocyst(P<0.05).EGF at a dose of 0.1 μg/L increased the total cell numbers of blastocyst compared with EGF at a dose of 0 μg/L(P<0.05).EGF at a dose of 100 μg/L had the lowest rate of the total cell numbers of blastocyst(P<0.05).Conclusions: ①IGF-Ⅱ(1,10 μg/L),glucose and insulin showed no significant effect of suppression or promotion among.②EGF(1,10 μg/L) in CZB medium with glucose,insulin and IGF-Ⅱcould involves in the regulationof differentiation of embryonic cells but no proliferation.③IGF-Ⅱ and EGF at a dose of 0.1 μg/L is best for cell culture concentration.④IGF-Ⅱ and EGF at a dose of 100 μg/L have inhibition effect on embryonic cells.更多还原