【摘要】 以蝴蝶兰嫩叶、茎尖、花梗侧芽、花梗节间为外植体,进行原球茎诱导,探求蝴蝶兰组织培养的最适外植体;通过不同培养基及各种激素配比组合进行原球茎诱导、增值及试管苗生根试验,探索各阶段最优培养基配方。结果表明:茎尖和花梗腋芽是蝴蝶兰形成原球茎的较佳外植体;M S+0.5 mg/LN AA+1.0 m g/L 6-BA+100 mL/L椰乳是蝴蝶兰原球茎增殖的最佳培养基,增殖系数达8.98;1/2 MS培养基最利于侧芽萌发形成丛生芽;生根最适培养基为1/2M S+1.0 mg/LIBA+0.5 mg/LN AA。更多还原

【Abstract】 Picking tender leaves,shoot-tips,pedicel lateral bud,pedicel internode as explants to induce original corm,to search the best explants on tissue culturing of Phalaenopsis amabilis;Different culture medium with hormone combination were screened to original corm induction,proliferation and rooting of seedling in test tube;Searching the optimal medium in different culturing stage.The result showed:the shoot-tips and pedicel lateral buds are the best explants for inducing original corm;The best culture medium of proliferation for original corm is MS+0.5 mg/L NAA+1.0 mg/L 6-BA+100 ml/L coconut milk,proliferation ratio is 8.98;1/2MS culture medium is the best to induce lateral bud forming clump buds;The optimum culture medium for rooting is MS +1.0 mg/L IBA +0.5 mg/L NAA.更多还原