【摘要】 制备了四种由β-四(3,5-二羧基苯氧基)酞菁锌(记为1)和β-八(3,5-二羧基苯氧基)酞菁锌(记为2)与人血清白蛋白(HSA)或牛血清白蛋白(BSA)构成的共价结合物,结合物中酞菁与白蛋白的摩尔组成比为6~7∶1。在PBS溶液中的电子吸收光谱测试表明,当酞菁1共价结合到白蛋白上后,呈现出更明显的单体特征吸收(单体特征吸收峰位于677 nm);而酞菁2在白蛋白网络中,主要以有利于光动力治疗的单体形式存在,最大吸收波长位于681 nm附近,摩尔吸光系数分别为2.01×105mol-1.L.cm-1(2-BSA)和2.05×105mol-1.L.cm-1(2-HSA)。两种酞菁被固定在白蛋白上后,在水溶液中的Q带吸收光谱和存在状态不随体系pH值的变化而改变,这与相应的游离酞菁明显不同。更多还原

【Abstract】 Four kinds of covalent serum albumin(BSA or HSA) conjugates of β-tetrakis[(3,5-dicarboxy)phenoxy]-phthalocyaninatozinc(1) and β-octakis [(3,5-dicarboxy)phenoxy]-phthalocyaninatozinc(2) were synthesized by the approach of amide bond.The molar ratio of phthalocyanine to albumin in conjugates were found to be 6~7∶1.Their absorption spectra were measured in PBS solution.When conjugated to albumin,compound 1 displays more distinct monomer absorption characteristics(with the maximum absorption at 677 nm) than its free form.Compound 2 in albumin framework exists mostly in monomer form,which is beneficial to photodynamic therapy in aqueous solution.Both 2-BSA and 2-HSA show a sharp and intense Q-band at 681 nm and 682 nm with the molar extinction coefficient of 2.01×105 and 2.05×105 mol-1·L·cm-1 respectively.The Q-band absorption spectra and existent state of phthalocyanine 1 or 2 in conjugates were not affected by the pH value of aqueous solution,which are obviously different from that of the corresponding free phthalocyanine.更多还原