【摘要】 目的:分析天水地区献血者血小板血型1～16抗原基因多态性,找出有临床意义的血小板抗原,建立本地区HPA血型库。方法:采用SSP-PCR法对天水地区172名血小板献血者进行HPA-1～16抗原32个等位基因检测分析,并与不同人群进行比较分析。结果:已检的样本中,通过Hardy-Weiberg吻合度检验,P>0.05,即期望的基因频率和检测到的基因频率是吻合的。其中以HPA-2、3、15最为复杂,各种基因型(a/a、a/b、b/b)均能见到;HPA-5、6的杂合子基因型较少见,分别为2例和4例;HPA-4、7、8、9、10、11、12、13、14、16均表现为单倍体型。结论:天水地区人群中,HPA-2、3、15最为复杂,抗原暴露率高,较易产生抗体,可为临床血小板输注提供免疫学依据。同时据此建立了天水地区人群的HPA血型库。更多还原

【Abstract】 Objective:To analyze 1～16 HPA antigen gene polymorphisms for blood platelet donors,identify platelet antigen with clinical significance,and establish HPA blood type library in the Tianshui area.Method:By SSP-PCR method in the Tianshui area,32 alleles of 172 platelet donors for HPA-1～16 antigen detection were analyzed and compared with different groups of people.Result: In the samples seized,the expected gene frequencies and gene frequencies detected are consistent by fit test of Hardy-Weiberg goodness(P>0.05).The HPA-2,3,15 is most complex,a variety of genotype(a /a,a/b,b/b) can been seen,but heterozygous genotypes of HPA-5,6 are rare,respectively 2 cases and 4 cases.HPA-4,7,8,9,10,11,12,13,14,16 showed haplotypes.Conclusion:In Tianshui area populations,HPA-2,3,15 is most complex because of high antigen exposure that easily produces antibodies,which can provide clinical immunological basis for platelet transfusion.Meanwhile HPA blood librariy was established in Tianshui area.更多还原