【摘要】 采用响应面法优化了超声提取熟地黄中5-羟甲基糠醛的工艺。选取提取时间、溶剂浓度和温度3个因素为随机因子,在单因素的基础上进行3因素3水平Box-Behnken中心组合试验设计,以5-羟甲基糠醛得率为响应值进行响应面分析。优化后的超声提取熟地黄中5-羟甲基糠醛的条件为:在提取时间61.6min,溶剂体积分数90.5%,提取温度为53.9℃,5-羟甲基糠醛得率理论值为0.3403%,验证值为0.3389%,两者间的相对误差为0.4%,验证值与理论值相吻合,说明该优化方法可行。更多还原

【Abstract】 Ultrasonic extraction of 5-hydroxymethylfurfural(5-HMF) from steamed Rehmanniae Radix was optimized by response surface methodology.Three factors three levers Box-Behnken design was employed to optimize the extraction process including extraction time,solvent concentration and extraction temperature as the three random factors.The extraction conditions after optimization were as follows:extraction time of 61.6min,methanol concentration of 90.5% and extraction temperature of 53.9℃.The relative error between the predictive yield(0.3403%) of 5-HMF under optimized conditions and results of the verification experiments(0.3389%) was only 0.4%,which indicated that the optimized method was feasible.更多还原