【摘要】 采用溶剂提取法提取大蒜中的活性成分,探讨了不同酶解温度和大蒜捣碎粒径程度对大蒜素提取率的影响。通过正交试验,研究了硫酸钡吸光比浊法测定大蒜中大蒜素含量的最佳实验条件。在最佳实验条件下,体系的吸光度y与硫酸根离子浓度x呈线性关系:y=0.237x+0.002(r=0.9985)。用MTT法对大蒜素提取浓缩液进行细胞活性检测,结果表明,大蒜素提取液能够有效抑制细胞增殖。更多还原

【Abstract】 Solvent extraction method was used to extract allicin.The influence of various hydrolysis temperatures and mashed garlic size on the extraction rate of allicin was discussed.The conditions of BaSO4 turbidimetry for measuring the content of allicin in garlic were optimized by orthogonal method.The absorbance(y)was proportional to the concentration of SO2-4(x),y=0.237x+0.002,with a correlation coefficient of 0.9985 under optimal experimental conditions.Cell activity was determined for allicin extract by MTT method,and the results showed that allicin extract can effectively inhibit cell proliferation.更多还原