【摘要】 目的探讨色素上皮衍生因子(PEDF)在成纤维细胞中的表达以及钙离子和氯化钴对其表达的影响。方法 19例包皮标本根据年龄分为2组:13例40岁以下者为I组,6例40岁以上者为II组。以I型胶原酶分离真皮成纤维细胞,37oC5%CO2培养。以1.0mmol/L氯化钙和100μmol/L的氯化钴作用于培养的成纤维细胞。以免疫荧光法检测PEDF蛋白质的表达,以逆转录-聚合酶链反应(RT-PCR)检测PEDF mRNA的表达情况。结果 PEDF表达于正常成纤维细胞,而且在组I和组II间mRNA相对表达量无差异性(分别为,P>0.05)。1.0mmol/L氯化钙和100μmol/L氯化钴显著增加PEDF mRNA相对表达量,分别为2.3倍和1.4倍,二者差异具有显著性(P<0.05)。结论真皮成纤维细胞表达PEDF,而且受钙离子和氯化钴的调节。更多还原

【Abstract】 Objective To investigate the expression of PEDF and its regulation by calcium and cobalt chloride(CoCl2) in dermal fibroblasts.Methods Ninteen normal human foreskin specimens were grouped into I or II according to their ages below(n=13) or above 40(n=6).Dermal fibroblasts were separated by collagenase type I and cultured at 37oC in 5% CO2.Calcium at 1.0 mM and CoCl2 at 100μM were incubated with fibroblasts for 24h.The mRNA and protein level of PEDF was determined by RT-PCR and immunofluorescence respectively.Results The expression of PEDF in normal fibroblasts was identified by RT-PCR and immunofluorescence,and no difference of PEDF mRNA was detected between groups I and II(P>0.05).Calcium at 1.0 mM and CoCl2 at 100μM significantly enhanced the expression of PEDF in cultured fibroblasts about 2.3 and 1.4 fold,respectively(P<0.05).Conclusion PEDF is expressed in dermal fibroblasts and is regulated by calcium and CoCl2.更多还原