【摘要】 目的体外分离培养流产4h、八月龄男胎的骨髓间充质干细胞(BMSCs),并观察BMSC在精原细胞培养条件下的生物学特征,探讨人骨髓间充质干细胞诱导分化为精原细胞的可行性,为BMSCs作为"种子细胞"开辟新的途径。方法分离培养、鉴定人BMSCs,研究其在精原细胞培养条件下诱导培养BMSCs并观察其生物学特征。结果 BMSCs具有间充质细胞的特征;实验组细胞形态发生变化,免疫组化显示Oct-4和C-kit染色阳性;对照组细胞则无。结论人胎儿BMSCs有较强的增殖能力;人胎儿BMSCs在精原细胞培养条件下诱导培养,能表现出精原细胞的一些生物学特征。更多还原

【Abstract】 Objective To isolate,purify and culture bone marrow mesenchymal stem cells(BMSCs) of aborted,8-month-old human male foetus and observe the main biological characteristics of BMSCs cultured in conditions for spermatogonia in vitro,so as to explore the possibility of differentiating BMSCs into spermatogonia as a new way to use BMSCs as " seed cells".Methods The BMSCs were purified,cultured in conditions for spermatogonia in vitro,and identified.Results Derived BMSCs exhibited the characteristics of mesenchymal cells,having an active proliferative ability in primary and passage cultures.The shapes of BMSCs changed and immunohistochemical staining for C-kit and Oct-4 was positive in the experimental group;but both were just the opposite in the control group.Conclusion Fetal bone marow-derived BMSCs has an active proliferative ability in primary and passage subcultures.BMSCs cultured in conditions for spermatogonia may show some biological characteristics of spermatogonia.更多还原