Objective To study on the metabolism of rutin by human intestinal bacteria in vitro.Methods Human intestinal bacteria was cultured in the GAM medium in vitro under the anaerobic environment at 37 ℃ condition.Rutin was put into the incubated buffer with inactivated human intestinal bacteria in control group,and with active bacteria in metabolic group.TLC and HPLC were used to analyze rutin and its metabolites.Results In control group,the content of rutin decreased by 34.2% gradually within 24 h,however,in me...