【摘要】 枯草芽胞杆菌R31在田间和温室对香蕉枯萎病表现出一定防效,为探索其防效产生的机制,利用耐受300μg/mL利福平和遗传稳定的突变株R31*测定其在巴西蕉和广粉一号粉蕉根际的定殖能力,进行盆栽灌根接种试验。结果显示,每株苗接种50 mL 1.78×108 CFU/mL的菌液,接种后60 d,标记菌株在香蕉根际土、根表、根内和球茎中的含量分别为4.14×106 CFU/g(干土)、1.09×107 CFU/g(鲜重)、4.66×104 CFU/g(鲜重)、1.23×103 CFU/g(鲜重);同样处理的粉蕉40 d后,根际土和根表的菌量分别为9.87×105 CFU/g(干土),7.9×104 CFU/g(鲜重)。表明突变株R31*在根际土和根表能成功定殖,但在根内和球茎的定殖量则较少,且根际土和根表的定殖量与接种浓度和每天最高气温有关。更多还原

【Abstract】 Bacillus subtilis R31 showed a certain control effect on banana panama disease in field and greenhouse.In order to explore its bio-control mechanism,a mutant R31* which can tolerance rifampicin at a rate of 300 ug/ml was selected to test the colonization ability on two banana varieties which were Musa spp.AAA var.brazil and Musa spp.ABB var.Guangfen No.1.Per banana seedling inoculated 50 ml 1.78×108 CFU/mL R31*,60 d after inoculation,the marked strains in banana rhizosphere soil,root surface,root and corm were 4.14×106 CFU/g(dry),1.09×107 CFU/g(fresh weight,FW),4.66×104 CFU/g(FW) and 1.23×103 CFU/g(FW),respectively.Similarly,40 d after inoculation R31* in Guangfen No.1,the number of R31* on rhizosphere soil and root surface were 9.87×105 CFU/g(dry) and 7.9×104 CFU/g(FW),respectively.It was found that R31* could colonize the rhizosphere soil and root surface of banana successfully,but the colonization quantity is less than rhizosphere in root and corm.And the population of R31* in rhizosphere soil and root surface have a relationship with inoculation concentration and daily maximum temperature.更多还原