【摘要】 研究了滇重楼种子休眠的解除方法以及外植体处理、培养条件对其幼苗生长发育的影响。结果表明,滇重楼种子在连续经过4℃低温层积4~5个月、红外光照射30 min、二次变温(4℃-40℃-20℃)处理、混合液(19 mmol/L KNO3+800 mg/L GA3)保湿7d后,休眠能得到明显解除,播种在营养钵(珍珠岩:蛭石=1:1)中60 d,萌发率可达40%;对胚根长至2 cm的萌发种子进行HgCl2消毒的最合适时间是10 min,既能控制污染率又不伤及胚芽的活性;在22℃、每天光照12 h(1 000 lx)的条件下,去除胚乳的幼芽在附加0.5 mg 6-BA和0.5 mg IAA的MS培养基上培养30 d后即可长成具有单叶的完整植株。更多还原

【Abstract】 In this study,we mainly focused on the methods for the breaking of Paris polyphylla var.yunnanensis seed dormancy,and further investigated the effects of explants treatment and culture conditions on the development and growth of its young seedlings.The results showed that the dormant seeds of Paris polyphylla var.yunnanensis were stimulated significantly after a series of treatments including continuous storage under 4 ℃ for 4 to 5 months,irradiation by infrared light for 30 minutes,treatment of fluctuating temperature twicely(4℃-40℃-20℃),kept in moisture in the complex liquid(19 mmol/L KNO3+800 mg/L GA3)for 7 day,and a germination rate as high as 40% was achieved when these treated seeds were grown for 60 days.When the germinated seeds with 2 cm long radicles were sterilized with HgCl2 for 10 min,it did not bring about high contamination rate nor a damage to the embryo buds.Cultured under conditions of 12 h illumination(1 000 l x) and 22℃ for 30 days,a complete young seedling with one piece of leaf was developed from the germinated seeds without endosperms on the MS medium supplemented with 0.5mg 6-BA and 0.5mg IAA.更多还原