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温度对西芹黄萎病病原菌生长和繁殖影响的研究
Studies on growth and reproduction of celery yellow wilt pathogenic fungi Fusarium oxyporum f.sp.apii on different temperature
【摘要】 田间流行病学调查发现,气温高于25℃时,西芹黄萎病容易发生和流行。为了解温度对西芹黄萎病流行的影响,室内研究了不同温度下西芹黄萎病病原菌(Fusarium oxyporum f.sp.apii,FOA)的生长和繁殖情况。结果显示,PDA培养的FOA菌丝在25℃时生长最好;埋放在土壤中的FOA小型分生孢子在35℃时萌发率最高;利用土壤浸提液培养的FOA在25℃时菌丝生长速度显著高于其他温度,总孢子产量在30℃和35℃时显著高于其他温度,小型分生孢子在高于25℃时是最主要的孢子。温度高于25℃的确有利于FOA的生长、繁殖,有利于病害在田间流行,与流行病学调查的结果吻合。表明温度是影响西芹黄萎病流行暴发的关键生态因子。
【Abstract】 Research of epidemiology in field showed that celery yellow wilt caused by Fusarium oxyporum f.sp.apii(FOA) was prevalent above 25℃.For finding out the relationship between epidemiology of celery yellow wilt and temperature,this study tested the growth and reproduction of FOA in different temperature.The results showed that,the best temperature for the mycelia growth in PDA medium was 25℃,the microconidia sprouted best on 35℃ in soil.When FOA was cultured in soil leach liquor medium,the best temperature for the mycelia growth was also 25℃,the total quantity of spores on 30℃ and 35℃ were higher than other temperature,and microconidia was the main spores form when temperature was above 25℃.The temperature above 25℃ was really advantageous for growth and reproduction of FOA,and these results were coincided with the research of epidemiology in field.Overall,temperature was critical ecological factor for outbreak and epidemic of celery yellow wilt.
【Key words】 celery yellow wilt; Fusarium oxysporum f.sp.apii; temperature; growth and reproduction;
- 【文献出处】 广东农业科学 ,Guangdong Agricultural Sciences , 编辑部邮箱 ,2011年11期
- 【分类号】S436.36
- 【被引频次】1
- 【下载频次】78