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低温对香樟膜脂过氧化和保护酶活性的影响

Effect of low temperature stress on membrane lipid peroxidation and protective enzyme activity of Cinnamomum camphora

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【作者】 孙存华孙存玉张亚红施剑杰孙东李百伟王丹蒋继宏

【Author】 SUN Cun-hua1,2,SUN Cun-yu1,3,ZHANG Ya-hong1,SHI Jian-jie1,SUN Dong1, LI Bai-wei1,WANG Dan1,JIANG Ji-hong2(1.College of Life Sciences,Xuzhou normal university,Xuzhou 221116,China;2.Key Lab of Biotech ndogy for Medicinal Plants of Jiangsu Province,Xuzhou 221116,China;3.Guiding Station of Suining County,Suining 221200,China)

【机构】 徐州师范大学生命科学学院江苏省药用植物生物技术重点实验室睢宁县林业指导站

【摘要】 以徐州引种驯化的成年香樟树叶片为材料,采用模拟低温胁迫法(温度处理分别为0℃、-5℃、-10℃、-15℃、-20℃、-25℃),研究了低温对成年香樟膜脂过氧化反应和保护酶活性的影响。结果显示,随着处理温度的降低,其叶片细胞质膜相对透性呈"S"型曲线增大,用Logistic方程计算其半致死温度为-13.40℃。叶片中MDA不断增加,SOD活性、POD活性、游离脯氨酸含量及可溶性糖含量随着处理温度的降低,呈先升后降的变化趋势。

【Abstract】 The effect of low temperature stress on membrane lipid peroxidation and protective enzyme activity in leaves of Cinnamomum camphora were studied with simulation low-temperature stress method(testing temperature was dropped from 0℃ to-25℃ gradually)in this paper.The results showed that the plasma membrane relative permeability in leaves of Cinnamomum camphora increased like a S-curve as the decreasing of temperature.Logistic equation showed that the semilethal temperature was-13.40℃.The content of MDA in the leaves increased,whereas proline,soluble sugar,the activity of SOD and POD in the leaves increased at first and then decreased as the temperature decreased.

【基金】 徐州师范大学研究生科研创新项目(09YLB033);国家林业科技支撑计划项目子专题(2006BAD03A00-6)
  • 【文献出处】 广东农业科学 ,Guangdong Agricultural Sciences , 编辑部邮箱 ,2011年04期
  • 【分类号】S792.23
  • 【被引频次】20
  • 【下载频次】182
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