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白皮松天然群体遗传多样性的等位酶分析

Allozyme Analysis of Genetic Diversity between Natural Populations of Pinus bungeana

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【作者】 丁小飞杨桂芬董梅童国富樊哲英王丹

【Author】 Ding Xiaofei1 Yang Guifen2 Dong Mei1Tong Guofu2 Fan Zheying3 Wang Dan1(1.Center for Forest Tree Breeding of Hubei Province,Wuhan,430079;2.Forestry Bureau of Nanzhang County;3.Jiangxia Vocational Technology School)

【机构】 湖北省林木育种中心湖北省南漳县林业局武汉市江夏职业技术学校

【摘要】 采用8种等位酶对白皮松4个天然群体的遗传多样性和遗传分化进行了研究。在4个群体中共检测到10个基因位点,15个等位基因,其中6个位点为多态位点;群体总体水平多态位点比率P=60%,平均有效基因数A=1.92,平均期望杂合度He=0.106,种群平均遗传距离为0.006 8。南漳白皮松群落平均等位基因数A=1.9,平均有效等位基因数Ae=1.08,多态位点百分率P=60.0%,期望杂合度He=0.092,观测杂合度Ho=0.098。南漳白皮松群体遗传多样性偏低,遗传变异水平较低,适宜采取原地保护和异地保存相结合的保护策略。白皮松4个群体的遗传分化系数Fst=0.005 7,基因流Nm=43.977 3,表明白皮松群体间有一定程度的遗传分化。聚类分析表明,4个群体分为3组,南漳群体与山西孝义群体的遗传距离最小,亲缘关系最近。

【Abstract】 Genetic diversity and genetic differentiation among four natural populations of Pinus bungeana were studied using eight allozyme systems.The results showed that 10 loci and 15 alleles were detected,of which 6 loci were exhibited polymorphism.Percentage of polymorphic loci P=60%,mean number of effective alleles per locus A=1.92,mean expected heterozygosity He=0.106,mean genetic distance were 0.006 8.The parameters of genetic diversity of P.bungeana of Nanzhang was given as follows:mean number of alleles per locus A=1.9,mean number of effective alleles per locus Ae=1.08,percentage of polymorphic loci P=60.0%,expected heterozygosity He=0.092,directly counted heterozygosity Ho=0.098.The genetic diversity in natural population of Nanzhang P.bungeana was slight low,and its implication for conservation strategy was discussed.Genetic diversity between populations Fst=0.005 7,Gene flow Nm=43.977 3,P.bungeana populations that have a certain degree of genetic differentiation.Four populations were divided into three groups according to Nei′s genetic distance,Genetic distance is minimum between Nanzhang population and Shanxi population,the relationship is recent.

【基金】 国家科技支撑计划(项目编号:2006BAD13B07-08)
  • 【文献出处】 广东林业科技 ,Guangdong Forestry Science and Technology , 编辑部邮箱 ,2011年01期
  • 【分类号】S791.243
  • 【被引频次】9
  • 【下载频次】126
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