【摘要】 目的探讨原因不明复发性流产(URSA)患者蜕膜组织中Th17/Treg免疫平衡情况。方法应用流式细胞技术检测URSA患者和正常早孕者蜕膜组织中Th17和Treg细胞频率,应用real-time PCR方法检测转录因子RORc和Foxp3 mRNA水平。结果 URSA患者蜕膜组织中Th17细胞在淋巴细胞和CD4+T细胞中的频率显著高于正常早孕者(P<0.001),URSA患者蜕膜组织中Treg细胞在淋巴细胞和CD4+T细胞中的频率显著低于正常早孕者(P<0.001)。URSA患者蜕膜组织中RORc mRNA水平明显高于正常早孕者,而Foxp3 mRNA水平显著低于正常早孕者(P<0.001)。结论 URSA患者蜕膜组织中存在明显的Th17/Treg免疫失衡,可能是URSA发生的重要原因。更多还原

【Abstract】 Objective To investigate the imbalance Th17/Treg in deciduas of unexplained recurrent spontaneous abortion(URSA). Methods In URSA patients and health early pregnant subjects,Th17 and Treg cells frequencies were measured by flow cytometry;while RORc and Foxp3 mRNA levels were measured by real-time PCR. Results There was significant increase in decidual Th17 cell frequency and RORc mRNA level in URSA patients(P<0.001).Meanwhile,there was significant reduction in decidual Treg cell frequency and Foxp3 mRNA level in URSA patients(P<0.001). Conclusion The decidual Th17/Treg imbalance plays an important role in URSA.更多还原