【摘要】 目的观察突变的大肠癌细胞线粒体DNA(mtDNA)转染NIH3T3细胞后转染细胞mtDNA D-环突变特性。方法通过脂质体法(Lipofection 2000TM)将大肠癌细胞突变的mtDNA真核表达载体转染NIH3T3细胞,利用G418抗性筛选克隆细胞;用PCR法检测转染细胞线粒体突变情况。结果突变mtDNA导致转染细胞mtDNA的突变和多态性变化。结论突变的大肠癌mtDNA可致NIH3T3细胞mtDNA位点变化,但具体的机制和过程有待于进一步研究。更多还原

【Abstract】 Objective To investigate the mtDNA mutation in NIH3T3 cell,which was transfected with mutated mtDNA from colorectal cancer cell line. Methods Recombinant eukaryotic vector,expressing plasmid of the mutated mtDNA,was transfected into NIH3T3 cell using Lipofection 2000TM and screened by G418.The mutated mtDNA of transfected NIH3T3 cell was detected by PCR. Results Mutation and polymorphism was observed in NIH3T3 cell transfected with mutated mtDNA. Conclusion MtDNA mutations in colorectal cancer cell affects NIH3T3 cell mtDNA loci,though further study is required.更多还原