【摘要】 本研究以V型小麦恢复基因的近等基因系为基础材料,运用蛋白质组学技术对V-CMS及杂种F1黄化苗和幼穗时期线粒体蛋白质表达图谱进行了比较,分析了差异蛋白质点与CMS的关系。主要结果如下:(1)比较了黄化苗和幼穗线粒体蛋白质的表达图谱,鉴定了10个差异表达的蛋白质点,包括与能量代谢有关的F0-F1ATP酶复合物的亚基和一些功能未知的蛋白质;(2)比较了V-CMS及杂种F1线粒体蛋白质表达图谱,鉴定了12个差异表达的蛋白质点,能量代谢相关的F0-F1ATP酶复合物的ATP酶β亚基和NADH脱氢酶亚基J和其他一些未知蛋白。结果表明,线粒体蛋白质的表达具有组织性和时空性;线粒体能够反向调控核基因编码的蛋白质在线粒体内的堆积,V-CMS中不育基因的表达能够调控线粒体内能量代谢相关蛋白质的表达水平,小麦V-CMS败育的发生可能是一种过早的PCD过程。更多还原

【Abstract】 In this paper, with near isogenic lines of restoring gene as materials, proteomics techniques and methods are adopted to compared V-CMS of wheat and hybrid F1 mitochondrial proteome expression profiles, analyze the differential mitochondrial proteins. The major results are shown as follows: (1) Comparing etiolated seedlings with spikes mitochondrial protein expression profiles, and identifying ten differential mitochondrial proteins, including energy metabolism proteins about F0-F1 ATPase complex subunit and unknown proteins; (2) Comparing V-CMS with hybrid F1 mitochondrial protein expression profiles between wheat etiolated seedlings and spikes tissue. We identified twelve differential mitochondrial proteins, and energy metabolism proteins, for example, ATP synthase beta subunit, NADH dehydrogenase subunit J and unknown proteins. After analyzing the differential mitochondrial proteins, we presume that mitochondrial retrograde regulation of the accumulation of nuclear-encoded proteins in the mitochondrial proteome of wheat, V-CMS gene encode protein induces the expression level of enegy metabolism proteins in mitochondrial, V-CMS in wheat maybe associated with premature programmed cell death.更多还原