【摘要】 对脂肪酶在丙酮介质中催化香草醇和壬酸甲酯合成辣椒素酯的反应动力学进行了研究.以HPLC方法测定酶催化合成产物辣椒素酯的浓度,确定了反应初速度;采用双倒数作图分析法和产物抑制试验法研究了本合成反应的机制,结果表明辣椒素酯的酶催化合成是按乒乓反应机制进行的.研究还确定了酶反应动力学方程和Vmax、KmA、KmB的值,并对其进行了验证,结果表明动力学方程与试验结果拟合度达0.997.更多还原

【Abstract】 The reaction kinetics for synthesis of capsinoids by lipase-catalyzed transesterification of vanillyl alcohol and methyl nonanoate in acetone was investigated in this work.The concentration of capsinoids obtained in the reaction was detected by HPLC and then the initial velocity of the synthesis reaction was calculated.The inhibiting test of product was carried out with methanol added.The results revealed that the enzymatic-synthesis reaction follows the Ping-Pang reaction mechanism.The kinetic equation with corresponding Vmax,KmA and KmB was inferred by the double reciprocal plot.The fitting degree of equation model arrives at 0.997 to compare with the experimental data.更多还原