【摘要】 目的研究大鼠弥漫性轴索损伤(diffuse axonal injury,DAI)后大脑海马中热休克蛋白质(heat-shockproteins,HSP)70 mRNA的表达变化。方法采用RT-PCR扩增,扩增产物用琼脂糖凝胶电泳检验,观察DAI后大鼠海马中HSP70 mRNA的表达变化。结果损伤后4 h,大鼠海马中HSP70 mRNA的表达增加,24 h表达达高峰,48 h后表达减弱。结论大鼠海马中HSP70 mRNA表达可作为DAI诊断的参考指标。更多还原

【Abstract】 Objective To explore the changes of expression of heat-shock proteins(HSP) 70 mRNA in hippocampus of rats after diffuse axonal injury(DAI).Methods RT-PCR products of HSP70 mRNA were tested by agarose gel electrophoresis after RT-PCR amplification.The changes of HSP70 mRNA expression were observed in rat hippocampus after DAI.Results The expression of HSP70 mRNA in the hippocampus could be detected 4 h after DAI.It reached maximum at 24 h and declined after 48 h.Conclusion HSP70 mRNA in hippocampus of rats could be useful for diagnosis of DAI.更多还原