【摘要】 目的研究猪眼玻璃体液成分变化及其与死亡时间(postmortem interval,PMI)的关系。方法放血处死家猪后取带眼睑的眼球96只,随机分为24组,在避光、温度(15±2)℃、湿度(50±5)%条件下分别放置2～96h,采集玻璃体液,运用全自动生化检测仪、超高效液相色谱检测K+、Na+、Cl-及次黄嘌呤(hypoxan-thine,Hx)浓度;用SPSS统计软件检测数据进行统计分析。结果玻璃体液中K+、Hx的浓度与PMI呈线性相关,R2分别为0.767和0.793;K+和Hx两者浓度与PMI经二元线性回归分析,R2为0.866;玻璃体液中Na+、Cl-浓度与PMI无明显相关性。结论玻璃体液K+、Hx浓度变化可作为较为客观的推断PMI的参考指标,两者与PMI的二元回归方程推断死亡时间有更高的准确性。更多还原

【Abstract】 Objective To investigate the relationship between postmortem interval(PMI) and concentration changes of components in swine vitreous humor.Methods Ninety-six porcine eyes from swine dying from acute massive hemorrhage,being randomly divided into 24 groups,were stored in dark situation,at temperature of(15±2) ℃ and humidity of(50±5)% for 2-96 hours separately.The vitreous humor was collected.Concentrations of K+,Na+,Cl-and hypoxanthine(Hx) were analyzed by automatic biochemical analyzer and ultra performance liquid chromatograph(UPLC).The data were statistically analyzed by SPSS software.Results Linear regression analysis showed that concentrations of vitreous K+ and Hx were positively correlated with PMI(R2=0.767 and R2=0.793,respectively).Binary linear regression showed a higher correlation for K+ and Hx with PMI estimation(R2=0.866).PMI was not significantly correlated with vitreous Na+ and Cl-concentrations.Conclusion Vitreous K+ and Hx concentrations can be used as the objective markers for PMI estimation.The binary linear regression functions of vitreous K+ and Hx concentrations with PMI are more accurate for estimating the PMI.更多还原