【摘要】 探讨电磁脉冲辐射对小鼠睾丸血-睾屏障紧密连接蛋白Occludin表达的变化。采用场强为400 kV/m的辐射场对30只成年雄性Balb/c小鼠进行200次重复全身照射,另30只假照射。于辐照后1、7、l4、21和28d分别取小鼠睾丸组织制备石蜡标本或提取组织蛋白和mRNA,使用Real-time PCR、Western-blotting和间接免疫荧光检测Occludin的表达及其变化。结果显示:与正常对照组相比,辐照组小鼠睾丸中Ocludin mRNA和蛋白的表达水平在辐照后1、7和14 d均明显降低。提示电磁脉冲辐射能够降低血-睾屏障紧密连接蛋白Occludin的表达,直接导致小鼠睾丸血-睾屏障的损伤。更多还原

【Abstract】 The expression and significance of blood-testis-barriar tight junction protein as well as Occludin in the testis of mice was investigated after whole-body exposure to electromagnetic pulse(EMP).Thirty adult Balb/c mice were irradiated to the whole body with 400kV/m EMP and 200 repeat while other thirty adult Balb/c mice were sham irradiated in the same condition.Specimens were harvested to prepare paraffin-embedded slice or to extract tis-sue protein and mRNA on 1,7,14,21 and 28 days after irradiation.Changes in cellular localization and expression of Occludin were examined by Real-time PCR,Western-blotting and immuno-fluorescence method,respectively.The results showed that the mRNA and protein levels of Ocludin expression were significantly decreased on 1,7 and 14 days after irradiation compared with control groups.These results indicate that EMP can reduce the expression of blood-testis-barrier tight junction protein Occludin and induce the damage of blood-testis-barrier.更多还原