【摘要】 探讨转化生长因子-β(Transforming growth factor-β,TGF-β)超家族成员TGF-β1、TGF-β3和TGFβ-R1在电磁脉冲辐射后成年小鼠睾丸中的表达变化及其意义。采用场强为400 kV/m的辐射场对20只成年雄性Balb/c小鼠进行200次重复全身照射,另20只假照射,于辐射后1、7、l4、21和28 d分别取小鼠睾丸组织制备标本,免疫组织化学ABC法检测TGF-β1、TGF-β3和TGFβ-R1的表达及变化,并通过图像分析系统以及统计学软件对实验结果进行统计学分析。结果显示:与正常对照组相比,辐照组小鼠睾丸中TGF-β1、TGF-β3和TGFβ-R1的表达和定位发生了细胞和时相特异性变化,表达强度也发生了显著变化。结果提示TGF-β1、TGF-β3和TGFβ-R1及其介导的信号转导通路在小鼠睾丸辐射损伤及修复过程中可能发挥了重要作用。更多还原

【Abstract】 In order to explore the expression and significance of transforming growth factor-β(TGF-β) super-family members TGF-β1,TGF-β3 and TGFβ-R1 in the testis of mice after whole-body exposure to electromagnetic pulse(EMP),twenty adult Balb/c mice were irradiated with EMP of 400 kV/m for 200 times to the whole body and other twenty adult Balb/c mice were sham irradiated in the same condition.Specimens were harvested on 1,7,14,21 and 28 days after irradiation.Changes in cellular localization and expression of TGF-β1,TGF-β3 and TGFβ-R1 were examined by immunohistochemical ABC method.Quantitative analysis of the immunostaining was made by an im-age analysis system.In the seminiferous tubules,the expression or localization of TGF-β1,TGF-β3 and TGFβ-R1 in the irradiated groups were significantly different from the control groups.These results indicate that TGF-β1,TGF-β3,TGFβ-R1 and the mediated signal pathway may play an important role in the process of impairment and repair of mouse testis irradiated by EMP.更多还原