【摘要】 本文用微波法提取蛹虫草培养基和子实体中的虫草素及腺苷,并利用高效液相色谱法同时测定虫草素和腺苷。选择Inertsil ODS-SP柱(150×4.6mm,5μm)为色谱柱,乙腈-水为流动相,流速1.0mL/min,柱温35℃,二极管阵列检测器(DAD)检测,检测波长260nm,进样量10μL。在2～10μg/mL浓度范围内,虫草素和腺苷线性关系良好,其相关系数分别为0.9998和0.9992。虫草素和腺苷的回收率分别是63.30%～67.11%和63.97%～70.39%,相对标准偏差分别是0.56%～1.97%和0.94%～1.97%,检出限分别是0.19μg/mL和0.02μg/mL。更多还原

【Abstract】 Cordycepin and adenosine from the culture medium of cordyceps militaris and cordyceps were separated by microwave extraction.Their concentrations were determined by high performance liquid chromatography.The chromatographic separation was achieved on a Inertsil ODS-SP column(150×4.6 mm,5 μm),using acetonitrile-water as the mobile phase with a flow rate of 1 mL/min at 35℃,a diode array detector(DAD) with 260 nm and a injection volume of 10 μL.The calibration curves of cordycepin and adenosine were linear over the concentration range of 2～10 μg/mL,and the correlation coefficients were 0.9998 and 0.9992.The standard recoveries were 63.30%～67.11% and 63.97%～70.39% with the RSDs of 0.56%～1.97% and 0.94%～1.97%,and the LODs were 0.19 μg/mL and 0.02 μg/mL,respectively.更多还原