【摘要】 运用荧光光谱法研究了注射用头孢美唑钠(Cefmetazole Sodium,CS)与牛血清白蛋白(BSA)的相互作用。CS对BSA具有荧光猝灭作用,其猝灭方式为静态猝灭,求出了猝灭常数,结合常数及结合位点数。在297K和311K时用Stern-Volmer方程和热力学方程处理实验数据,得到了结合常数KA、热力学参数△H、△G、和△S。结果表明CS与BSA之间的作用力主要为氢键和范德华力。Zn2+、Cu2+对CS与BSA的结合常数和结合位点数均有影响,Zn2+浓度接近人体血浆中浓度时,能够促进CS与BSA的相互作用。更多还原

【Abstract】 The interaction between cefmetazole sodium for injection and bovine serum albumin(BSA) was studied by the fluorescence spectroscopy.The fluorescence of BSA was quenched by cefmetazole sodium for injection,and the combination was a static quenching process.The quenching data were analyzed according to Stern-Volmer equation,and the quenching constant,binding constant,binding sites and thermodynamic parameters were obtained.Furthermore,the interaction between cefmetazole sodium for injection and BSA was investigated in the presence of Zn2+ and Cu2+,and the results showed that the binding constant and binding site were enhanced by Zn2+.更多还原