【摘要】 基于快速、持续、无创检测技术在血糖监测领域的重要性,以聚甲基丙烯酸甲酯微球阵列为光子晶体制孔模板,以葡萄糖为印迹模板,N-异丙基丙烯酰胺和甲基丙烯酸羟乙酯为混合单体,4-乙烯基苯硼酸为识别基,N,N-亚甲基双丙烯酰胺为交联剂,在制孔模板间隙进行共价型分子印迹聚合,除去制孔模板后,制得具有规整孔结构的新型光学凝胶材料——分子印迹光子晶体。实验表明:此材料对葡萄糖响应速度快,选择性高,印迹聚合物对葡萄糖吸附量在3 min即达到3.2 mg/g。在同等条件下,印迹聚合物对葡萄糖的吸附量是其它类似物吸附量的2倍以上。印迹聚合物的识别行为以衍射光波长变化形式表现,随着葡萄糖浓度由0 mol/L增加到0.02 mol/L,葡萄糖分子印迹光子晶体的衍射波长发生150 nm的红移,表现出良好的传感特性。此材料为葡萄糖检测提供了新思路,有望用于人体泪液或尿液中葡萄糖浓度的检测,实现非侵入式的检测手段。更多还原

【Abstract】 A three-dimensionally ordered,macroporous,inverse-opal glucose molecularly imprinted polymer crystal(MIPC) film was synthesized by the inverted colloidal-crystal template technique.Preparation of our MIPC was carried out by thermo-poymerization of monomers(2-hydroxyethyl methacrylate and N-isopropylacrylamide),covalent recognizer(4-ethylenyl phenylboronic acid),and cross-linker(N,N′-Methylenebisacrylamide) with poly(methyl-methacrylate)(PMMA) particle array as template,followed by chemical removal of the template using acetone.Due to its 3D-ordered macroporous arrays with molecular imprinting,nanocavities,the formed photonic hydrogel allows rapid and selective detection of the target glucose.The glucose adsorption amount of our MIP was 3.2 mg/g in 3 min which is two times more than that of other analogues.The diffraction wavelength of the MIPC redshifts was more than 150 nm in response to 0.02 mol/L of glucose.Our results revealed that MIPC could provide a novel non-invasive approach for glucose detection in tears or urine and exhibit a promising potential for development of new glucose monitoring technology.更多还原