【摘要】 目的研究一次性使用牙刷上脱落细胞的DNA提取和STR分型。方法对一次性使用牙刷的采集方法、采集部位、DNA提取方法、存放时间对STR分型的影响进行比对研究。结果割取法可获得较高浓度的DNA,30例中检出9个以上基因座达27例,与擦拭法存在统计学差异(P<0.05)。Chelex-100法、DNA IQTM法检出9个以上基因座分别为26例、24例,STR分型结果无统计学意义(P>0.05)。提取牙刷的前、后部三束刷毛检出9个以上基因座分别达27例、28例,STR分型结果无统计学意义(P>0.05);放置1天、1周、1个月、3个月、6个月的时间后检出9个以上基因座分别为28例、27例、22例、12例、7例。结论割取法提取一次性牙刷上的脱落细胞进行STR分型效果良好;放置时间越长的牙刷,检出率越低。更多还原

【Abstract】 Objective To study the extract concentration and STR typing of exfoliated cells from disposable toothbrush.Methods Disposable toothbrushes were studied including different cell collection sites and methods,different DNA extraction methods,and the influence of different storage time on STR typing.Results The cutting method could get higher concentrations of DNA.In all 30 cases,more than 9 STR loci could be detected from 27 cases,and there was significant difference(P<0.05)compared with the swab method.26 and 24 cases were detected in more than 9 loci by the Chelex-100 and DNA IQTM methods respectively,and the difference was not significant(P>0.05).27 and 28 cases were detected in more than 9 loci by collecting 3 beam bristles from the front and rear of toothbrush respectively,and the difference was not significant.After storage 1 day,1 week,1 month,3 months and 6 months,the case number in which more than 9 loci could be detected was 28,27,22,12 and 7 respectively.Conclusion Cutting the toothbrush can get better STR typing results than the swab method.The longer the toothbrush placed,the lower detection rate.更多还原