【摘要】 目的:通过检测乳腺针吸细胞中的生存素(survivin)表达水平,为乳腺癌的临床早期发现、术后复发监测,提供方便、快捷的方法。方法:收集乳腺癌患者和其他乳腺疾病以及健康志愿者的乳腺针吸细胞提取其总RNA,采用逆转录-巢式聚合酶链反应检测生存素基因表达水平,同时采用免疫组化进行病理分级。结果:185例乳腺癌患者针吸细胞中生存素基因阳性率90.8%,52例其他乳腺系疾病的阳性率为1.9%,30名健康志愿者阴性,乳腺癌患者针吸细胞中生存素基因表达量在1、2级与3级间有显著性差异(P<0.01)。结论:RT-nest-PCR法检测乳腺针吸细胞中生存素基因可以作为早期诊断、术后复发监测乳腺癌的指标。更多还原

【Abstract】 Objective To establish a convenient and guid method for early diagnosis,prediction and routine screening of breast cancer through detecting the expression of survivin gene in mammary gland piqure cells.Methods Mammary gland piqure cells were collected from breast cancer patient,other mammary gland disease patient and healthy individuals.Then the total RNA of each sample was extracted.The Rt-nest-PCR was established for detection of survivin gene and the breast cancer tissue were classified pathology with immunohistochemistry.Results Sensitivities of the assay are 90.8% in 185 breast cancer,1.9% in other patients and 0% in normal person respectively.The expression of survivin between G1,G2 and G3 breast cancer tissues have very marked statistic significance(P<0.01).Conclusion Detection of the survivin mRNA by RT-nest-PCR can be utilized as an indicator of early diagnosis and prediction of breast cancer.更多还原