【摘要】 目的:应用化学发光法、酶联免疫吸附试验(ELISA)定量检测类风湿性关节炎(RA)患者抗环瓜氨酸多肽(CCP)抗体的含量,探讨CCP抗体在RA早期诊断和治疗中的作用。方法:选取69例RA、30例强直性脊柱炎(AS)、33例干燥综合征(SS)患者、61例健康体检人群,分别用化学发光、ELISA检测其血清CCP抗体的含量。结果:两种方法检测193例样本CCP抗体,阳性结果符合率为98.08%,阴性符合率为100%,总符合率为99.48%,CCP抗体对RA诊断的敏感性、特异性分别为(69.56%,68.11%)、(96.77%,97.26%)、RA患者活动期CCP抗体含量(141.10±93.95)IU/m l与非活动期(45.14±10.54)相比,有显著性差异(P<0.05)。结论:化学发光、ELISA两种方法定量检测CCP抗体的符合率良好,化学发光法适用于仪器自动化,操作简便,易于标准化,可满足临床的需要。CCP抗体是一种新的对RA有较高诊断价值的血清学标志物,有助于对RA病情监测。更多还原

【Abstract】 Objective To evaluate the sensitivity and specificity of anti-cyclic citrullinated peptide antibody(anti-CCP) in patients with rheumatoid arthritis(RA).Methods Anti-CCP antibodies were examined by using MEIA and ELISA from 69 RA,30 Ankylosingspondylitis(AS),33 Siccasyndrome(SS) and 61 normal donors sera.Results Among 69 patients with RA,The sensitivity and specificity of anti-CCP were(69.56%,68.11%),(96.77%,97.26%) by MEIA and ELISA in RA respectively.The data showed that there was a significant difference between periods of activity and periods of quiescence,However,no difference was found between the results in RA by using MEIA and ELISA.Conclusion Detecting anti-CCP by MEIA may be a brief,standard and reliable method.It might present more sensitive and specific in clinical diagnosis on RA.更多还原