【摘要】 采用cDNA末端快速扩增方法,获得了番木瓜果实GDP-D-甘露糖焦磷酸化酶(GMP)基因的cDNA全长序列,将其命名为CpGMP,GenBank登录号为FJ489652.然后,根据拼接序列设计开放性阅读框上、下游引物,扩增得到了CpGMP基因的开放性阅读框序列和DNA序列.ORF序列编码361个氨基酸,DNA序列包含4个外显子和3个内含子.序列分析表明,番木瓜GMP与大果黄褥花、桃、番茄、拟南芥、水稻GMP的同源性分别为90.30%、89.47%、88.92%、88.09%、85.87%.半定量RT-PCR分析表明,CpGMP基因随着番木瓜果实的成熟表达量逐渐增加,软化时又逐渐降低.更多还原

【Abstract】 The full-length GMP cDNA was cloned by RACE-PCR from papaya and the gene w as designated as CpGMP(GenBank accession No.FJ489652).Open reading frame which encoded 361 amino acids was cloned from cDNA.DNA sequence including 4 extrons and 3 introns was cloned from genomic DNA.Sequencing analysis showed that the amino acid sequence was 90.30%,89.47%,88.92%,8 8.09%,85.87% homologous with GMP from Malpighia glabra,peach, tomato,Arabidopsis thaliana and rice,respectively.Semi-quantitative RTPCR analysis showed that the expression of CpGMP increased gradually with pa paya fruit maturating and decreased when fruit began to soften.更多还原