【摘要】 抑郁症是一种以情绪低落为主要症状的情感性精神障碍.利用慢性不可预知温和应激建立抑郁症小鼠模型,以蔗糖水消耗实验作为抑郁核心症状之一———快感缺乏的检测方法.将4周龄雄性小鼠(C57BL/6)持续6周暴露于一系列轻度应激中,每周检测蔗糖水消耗.应激包括:令小鼠恐惧的气味、噪音、饲养笼倾斜、潮湿的垫料、无垫料的新饲养笼、照明过夜以及短暂的同笼饲养.前3周模型组每天随机应用2个不同的应激,后3周应用3个不同的应激.结果:随着应激强度的增加,模型组对蔗糖水的摄取量显著低于对照组.由此表明,慢性不可预知性温和应激能导致小鼠出现对奖赏的反应性下降,快感缺乏等抑郁症状.更多还原

【Abstract】 Depression is a chronic mental disorder mainly characterized by depressed mood.A series of chronic unpredictable mild stresses(CUMS) was used to set up a mice model of depression.And sucrose consumption test was adopted to estimate the anhedonia,one of the core symptoms of human depression.Mice(C57BL/6) were exposed to CUMS over 6 weeks,and anhedonia was evaluated by weekly monitoring sucrose intake.The stressors included that soiled cage with aversive odour,noise,cage tilt,damp bedding,illumination overnight,paired-housing.Week 1—3,two stressors was used daily.Week 4—6,three stressors was used.The results showed that,with the increasing of the stress frequency,sucrose consumption of the stress group was statistically decreased compared with the control group.This indicated that CUMS causes a decrease in responsiveness to rewards,which reflected the key symptom of depression-anhedonia.更多还原