【摘要】 2010年7月某养牛场肉牛开始发病,临床症状表现为食欲下降,流鼻,体温升高,呼吸困难等,病程7 d至1月,病牛因极度虚弱而死亡。为查明病因并制定有效的综合防控措施,对其中5头病死牛肺和脾等内脏器官进行病理学观察、细菌分离鉴定以及药敏试验。病理组织学观察显示,肺表现为纤维素性大叶性肺炎病变,肺细支气管、肺泡腔内有大量中性粒细胞、纤维素以及少量的淋巴细胞、单核细胞浸润,肺泡壁毛细血管扩张充血;脾脏的脾小体减少甚至消失,红髓区严重出血,中性粒细胞浸润。从剖检的5头病死牛肺、心、脾脏中均分离到金黄色葡萄球菌、肺炎链球菌、牛链球菌和溶血性巴氏杆菌等4种病原菌。药敏试验结果显示,金黄色葡萄球菌对各类药物均高度敏感,肺炎链球菌和牛链球菌对青霉素类、头孢类和四环素类药高度敏感,溶血性巴氏杆菌对头孢噻肟、头孢哌酮和强力霉素高度敏感。综合病理变化以及病原菌的分离鉴定结果,表明因多重病原菌感染而引发牛严重的呼吸道疾病。更多还原

【Abstract】 There was a case of beef cattles died of extremily feeble in July 2010,the sick cattles showed loss of appetite,flowing blenna narium,fever and anhelation in clinical symptoms,disease keep on 7 days to one month.In order to search for the etiological factors and find out an effective integrated control measures,we observed the histopathological changes,and isolated,identified the pathogenic bacteria from lung,spleen and other organs of five cattles among those dead cases.The main histopathologic changes were fibrinous lobar pneumonia.There were many kinds of cells infiltrating in lung bronchioles and alveolar space,including a large number of neutrophils and cellulose,and a small number of lymphocytes and monocytes.At alveolar wall there were telangiectasis and hyperaemia.We also found the splenic corpuscle diminished or even disappear,heavy bleeding and neutrophils infiltrating in splenic pulp areas.Staphylococcus aureus,Streptococcus pneumoniae,Streptococcus bovis and Pasteurella multocida were isolated from lungs,hearts and spleens of all the five dead cattles.The results of drug susceptibility test showed that Staphylococcus aureus was hypersensitive to all drugs,Streptococcus pneumoniae and Streptococcus bovis were hypersensitive to penicillins,cephalosporins and tetracyclines,and Pasteurella multocida was hypersensitive to cefotaxime,cefoperazone and vibramycin.Based on pathological changes and isolation and dentification of pathogenic bacteria,it show that the sick cattles were infected by multiple pathogens and to cause serious respiratory disease.更多还原