【摘要】 根据病毒gag变异区的两端设计引物,PCR扩增ENTV及ERVs的gag变异区,TA克隆并测序,经比对分析发现,在ERVs的783位~803位的"CCT"重复序列,编码多聚脯氨酸"PPPPPPP",对应在ENTV gag中存在缺失突变和点突变,编码氨基酸为"PSL",生物信息学预测该区域的变异位于一个重要的抗原决定簇中,对gag在ENTV和ERVs中的功能有较大影响,为进一步研究gag的抗原性及相关免疫学检测方法的建立奠定基础。更多还原

【Abstract】 The RT-PCR/ PCR were carried out on nasal fluid from goats with enzootic nasal tumor(ENT) and on genome of ENT free goats,using primers designed from both sides of gag variable regions of enzootic nasal tumor virus(ENTV) and endogenous retrovirus(ERVs) of goats.The PCR fragments were purified,cloned into the pMD18-T vector and sequenced.The alignment of thsee fragments showed that defetion and point mutations were foundin the encoding "PSL" region in ENT corresponding to the repeated sequences "CCT"(nt 783-803) in ERVs eloding polgprolinerepeated sequences "CCT"(nt 783-803) in ERVs,which encoding polyproline "PPPPPPP".Bioinformatics analysis showed that variable region positioned in an antigenic determinant affects the biologic activity of gag.These results will help the research of antigenicity of gag and the establishment of immunoassay against ENTV infection.更多还原