【摘要】 目的检测干细胞转录因子Oct-4皮肤鳞状细胞癌(SCC)、脂溢性角化病(SK)的组织表达,分析其表达差异及与肿瘤干细胞的关系。方法采用免疫组织化学方法检测Oct-4在35例SCC、21例SK及15例正常对照皮肤组织中的表达。结果 Oct-4在正常皮肤组织表达为阴性,在SCC、SK之间表达有显著性差异(P<0.05),间接反映SCC、SK的恶性程度不同。结论 SCC、SK中表达Oct-4基因的细胞很可能是SCC、SK的干细胞,这将为其最终分离和鉴定人SCC、SK干细胞提供重要基础,将有助于揭示SCC、SK的肿瘤起始细胞,从而对预防和治疗SCC、SK有重大意义。更多还原

【Abstract】 Objective To detect the expression of stem cell transcription factor Oct-4 in squamous cell carcinoma(SCC) and seborrheic keratosis(SK) and its association with cancer stem cells. Methods Immunohistochemistry was used to detect Oct-4 expression in 35 SCC cases,21 SK cases and 15 normal control skin tissues. Results Oct-4 expression was negative in normal skin and showed a significant difference between SCC and SK tissues(P<0.05) . Conclusion The Oct-4-positive cells in SCC and SK are probably tumor stem cells. Oct-4 expression may provide an important evidence for isolation and identification of human SCC and SK stem cells.更多还原