【摘要】 目的研究红色毛癣菌对人角质形成细胞模式识别受体TLR-2,TLR-4,Dectin-1表达及细胞因子分泌的影响,探讨角质形成细胞对红色毛癣菌的免疫应答及其机制。方法红色毛癣菌孢子与人永生化表皮细胞株HaCaT细胞共培养,采用Real time PCR检测共培养后HaCaT细胞TLR-2,TLR-4,Dectin-1mRNA的表达情况;采用流式细胞技术检测共培养后不同时间段HaCaT细胞TLR-2,TLR-4及Dectin-1平均荧光强度;采用蛋白芯片抗体阵列检测共培养上清液中36种不同的细胞因子,趋化因子和急性时相蛋白的表达情况。结果共培养6 h后,TLR-2,TLR-4,Dectin-1mRNA表达上调;共培养量明24显h增后加TL。R结-2,论T L人R角-4,质D形ec成tin细-1胞平对均红荧色光毛强癣度菌增的高免,细疫胞识因别子和I应L-答8,,I在-30一9,定IF程N-度γ,上IL可-6通,IL过-1上3调在红Ha色Ca毛T癣细菌胞刺中激模后式分识泌别受体TLR-2,TLR-4及Dectin-1后分泌多种细胞因子来实现。更多还原

【Abstract】 Objective To investigate the effects of Trichophyton rubrum exposure on the expressions of toll-like receptor-2(TLR-2),TLR-4 and dendritic cell associated C-type lectin-1(Dectin-1) and cytokine secretions in human keratinocytes cell line HaCaT.Methods The mRNA of TLR-2,4,and dectin-1 in the HaCaT co-cultured with the conidia of Trichophyton rubrum conidia for 24 h was measured with real-time PCR.The mean fluorescence intensity and the percentage of cells positive for TLR-2,4,and dectin-1 was detected during the co-culture using flow cytometry.The cytokine secretion profiles in the cell culture supernatant was analyzed using a cytokine antibody array.Results The TLR-2,4,and dectin-1 mRNA expressions,mean fluorescence intensity and percentage of positive cells for TLR-2,4,and dectin-1 all increased in HaCaT cells in response to TTrichophyton rubrum conidia exposure.The results of cytokine antibody array demonstrated obviously increased secretions of IL-8,I-309,IFN-γ,IL-6,and IL-13 in the culture supernatant of HaCaT cells in response to TTrichophyton rubrum exposure.Conclusion The immune responses and immunological recognition of human keratinocytes to Trichophyton rubrum conidia are partially mediated by up-regulating the expressions of TLR-2,TLR-4 and dectin-1 and secretions of multiple cytokines.更多还原