【摘要】 中缅树鼩作为一种新型实验动物,在医学生物学上,尤其是病毒学方面的应用受到越来越多的重视。实验动物自身病毒感染会影响动物健康和干扰实验结果,甚至危害实验人员生命安全。所以,实验动物病毒检测一直是动物质量控制的重要部分。中缅树鼩研究迄今缺乏清晰的病毒自然感染资料。为调查中缅树鼩的病毒感染状况,采集野生俘获和人工繁殖的中缅树鼩血清样本272份,全部血清样本通过ELISA方法对乙型肝炎病毒(HBV)表面抗原,丙型肝炎病毒(HCV)总抗体,以及戊型肝炎病毒(HEV)、腺病毒(ADV)、单纯疱疹病毒1型(HSV-1)和2型(HSV-2)的IgG抗体进行了检测。结果表明,ELISA初筛HBV表面抗原有3份阳性样本,但通过乙型肝炎两对半定量检测进一步确认为阴性;抗HCV抗体和抗HEV、ADV、HSV-1IgG抗体检测均为阴性;抗HSV-2IgG检测有1份阳性样本。提示仅抗原或抗体血清学指标检测树鼩肝炎结果并不能反应个体携带病毒的状态,应该再进行病毒学指标确认。同时建议中缅树鼩繁殖群应进行HSV-2的筛选,以便杜绝和控制该病毒的感染。更多还原

【Abstract】 Tupaia(Tupaia belangeris chinensis,tree shrew) as a new experiment animal in medicine are non-rodent,small animals and close to primates in evolution.Experimental animals infected with viruses will affect the animal’s health,interference experiment,and even endanger the operator’s safety.Therefore,the viral infection in experimental animals has long been considered an important part of quality control.Lack of clearer viral natural infection information on the T.belangeris limits its use.Six viruses infection in 272 wild capture and artificial breeding Tupaia were investigated in this study.All serum samples were detected for the hepatitis B virus surface antigen,the total antibodies of HCV,hepatitis E virus(HEV),adenovirus(ADV),herpes simplex virus type 1(HSV-1) and herpes simplex virus type 2(HSV-2) by ELISA.The results showed that anti-HCV antibody and anti-HEV,ADV,HSV-1 IgG antibodies were negative,only one sample was positive of anti-HSV-2 IgG..Three samples were positive in the primary ELISA detection of HBV surface antigen,but two pairs of semi-quantitative detection of hepatitis B and further recognized as negative.The results implied that antigen or antibody-positive results appeared in the hepatitis serological test is not accurate enough and confirmation by other virological indicators is necessary.Tupaia breeding herd should be screened for HSV-2 in order to prevent and control the virus infection.更多还原