【摘要】 目的检测IL-17和STAT3在结直肠癌组织中的表达,探讨其与结直肠癌临床病理特征和肿瘤血管生成的关系。方法应用免疫组织化学S-P法检测94例结直肠癌组织和31例正常肠黏膜组织中IL-17、STAT3蛋白的表达,并应用CD34标记微血管计算MVD。结果免疫组化结果显示IL-17主要表达在结直肠癌组织中的肿瘤细胞和肠黏膜固有层淋巴细胞胞浆内,阳性表达率明显高于正常肠黏膜(P<0.01);IL-17的表达与结直肠癌分化程度和Dukes分期有关(P<0.05)。STAT3在结直肠癌组织中的表达显著高于其在正常肠黏膜中的表达(P<0.01),且与肿瘤细胞分化程度、淋巴结转移和Dukes分期有关(P<0.05)。IL-17和STAT3在结直肠癌组织中的表达与MVD有关(P<0.05)。Spearman等级相关分析表明IL-17和STAT3在结直肠癌组织中的表达呈正相关(r=0.251,P=0.015)。结论 IL-17和STAT3在结直肠癌组织中高表达,可能在结直肠癌的血管生成和肿瘤的发生、发展中起重要的作用。更多还原

【Abstract】 Objective To detect the expression of IL-17 and STAT3 in colorectal cancer(CRC) tissue and explore their relation with its clinicopathological features and angiogenesis.Methods Expression of IL-17 and STAT3 in 94 CRC tissue samples and 31 normal intestinal mucosa tissue samples was detected by immunohistochemistry SP method and microvessel density(MVD) was counted according to the number of CD34-marked microvessels.Results Immunohistochemistry showed that the IL-17 was mainly expressed in cytoplasm and lymphocytes of normal intestinal mucosa tissue samples,and its positive expression rate was significantly higher in CRC tissue samples than in normal intestinal mucosa samples(P<0.01).The expression of IL-17 was related with the CRC cell differentiation and Dukes stage(P<0.05).The expression level of STAT3 was significantly higher in CRC tissue samples than in its adjacent normal tissue samples(P<0.01),which was related to the CRC cell differentiation,lymphatic metastasis and Dukes stage(P<0.05).The expression of IL-17 and STAT3 in CRC tissue samples was related with MVD(P<0.05).Spearman stage correlation analysis showed that the expression of IL-17 was positively related with that of STAT3 in CRC tissue samples(r=0.251,P=0.015).Conclusion Over-expression of IL-17 and STAT3 in CRC tissue may play an important role in the angiogenesis and carcinogenesis of CRC.更多还原